ABSTRACT
The use of laparoscopic surgical techniques is now being applied to a variety of operations traditionally performed in an open fashion. The indication for surgery included polyps, obstruction, bleeding, and perforation. Small bowel perforation was usually treated with open surgery, but now, laparoscopic-guided bowel surgery is technically feasible and should translate into shorter hospitalization and less patient discomfort. Recently, we successfully treated a case of laparoscopic assisted suture of small bowel perforation. Here we report this case with a brief review of literature.
Subject(s)
Humans , Hemorrhage , Hospitalization , Polyps , SuturesABSTRACT
OBJECTIVE: Isoliquritizenin (ISL) is a chalcone flavonoid, present in licorice, shallot and bean sprouts, has cancer preventing properties and often used in chinese medicine. In this study, ISL to determine its effect on cell proliferation and cell cycle progression in human cervical cancer cells were evaluated. METHODS: Cell viability assay was carried out to determine the viability of human cervical cancer cells. We tested the several experimental methods for verification and functional identification, including MTT assay, FACS analysis, DNA fragmentation assay, and Western blot analysis for ISL treated human cervical cancer cells (HeLa). RESULTS: ISL, induced growth inhibition in a dose dependent manner, treatment with 50 microM/L ISL blocked 50% cell growth. FACS results showed that there was no change in the S phase, but on the other hand ISL increased the percentage of cells in G1 phase. DNA fragmentation assay by ELISA was done to find the rate of apoptosis. Apoptosis took place but in a reduced manner. From Western blot analysis, it revealed ISL induced the expression of p21(Cip1/Waf1) and p27(kip1) but not mediated by p53. Caspase pathway was revealed and cleavage of PARP took place. CONCLUSION: ISL, a chalcone flavonoid, inhibited cell proliferation and induced cell cycle arrest at sub G1 by enhancing the production of p21(Cip1/Waf1) and p27(kip1). These results indicate that ISL will be a promising agent for use in chemopreventive or therapeutic against human cervical cancer cells.
Subject(s)
Humans , Apoptosis , Asian People , Blotting, Western , Cell Cycle , Cell Cycle Checkpoints , Cell Proliferation , Cell Survival , Chalcone , DNA Fragmentation , Enzyme-Linked Immunosorbent Assay , G1 Phase , Glycyrrhiza , Hand , S Phase , Shallots , Uterine Cervical NeoplasmsABSTRACT
The estimated incidence of conjoined twins is one in 50,000 births, however, over 60% are stillborn, making the observed incidence around one per 200,000 live-births. Thoracopagus is the most common variety of conjoined twins accompanying with congenital heart disease 75%, fusion of alimentary tracts 50%, single external biliary tract 25%. Approximately 90% of thoracopagus conjoined twins are not candidates for surgical separation due to the high degree of cardiac union and the complexity of cardiovascular anomalies. With improvement in prenatal diagnosis and routine ultrasound monitoring of pregnancies, these cases are more commonly being diagnosed prenatally. Accurate diagnosis of the shared anatomy and associated malformations is crucial for counseling of the affected families regarding outcome as well as planning potential postnatal surgical separation. We experienced a case of thoracopagus with single conjoined heart that was diagnosed by ultrasonography at 15 weeks of gestation in 33 years old woman. So we present this case with a brief review of literatures.